Analysis Note

ControlMCF7 cell lysate

Application

Research CategorySignaling

Detect phospho-p70 S6 Kinase (Thr389) using this Anti-phospho-p70 S6 Kinase (Thr389) Antibody validated for use in Western Blotting, Peptide Inhibition Assay.

Research Sub CategoryKinases & Phosphatases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The p70 S6 kinase is a ubiquitous cytoplasmic protein that is activated in response to cytokines, and growth factors including the epidermal growth factor and fibroblast growth factor. It lies downstream of the mTOR/PI3K pathway and is phosphorylated on multiple residues: Thr389, Thr229, Thr421, Ser371, Ser404, Ser411, Ser418, and Ser424. Thr389, Ser371, and Ser404 are located in the linker domain; whereas Thr421, Ser411, Ser418, Ser424 are located in the autoinhibitory domain, and Thr229 is found in the catalytic domain. These sites may be phosphorylated in a hierarchical sequence leading to the activation of the p70 S6 kinase. The p70 S6 kinase phosphorylates the S6 protein on the 40S ribosomal protein (rpS6), and plays a role in multiple processes including regulation of mRNA molecules with 5'-terminal oligopyrimidine tracts, protein synthesis, cell growth, proliferation, and cell survival. The activity of this kinase may be important in diabetes, cancer, and aging.

Immunogen

KLH-conjugated linear peptide corresponding to the AGC-kinase C-terminal of human Ribosomal protein S6 kinase beta-1
phosphorylated at Thr389.

Epitope: AGC-kinase C-terminal

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot (Peptide Inhibition) Analysis in MCF7 cell lysate and MCF7 cell lysate with non-phospho or phospho peptide.

Western Blot (Peptide Inhibition) Analysis: 1.0 µg/mL of this antibody detected p70 S6 Kinase phosphorylated at Thr389 in 10 µg of MCF7 cell lysate and MCF7 cell lysate incubated with a non-phospho peptide.

Specificity

Other homologies: ribosomal protein S6 kinase beta-2 (91% sequence homology).

This antibody recognizes the AGC-kinase C-terminal of Ribosomal protein S6 kinase beta-1 phosphorylated at Thr389.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~70 kDa observed