Application

Research CategoryNeuroscience

Anti-SynGAP Antibody detects level of SynGAP & has been published & validated for use in IC & WB.

Immunocytochemistry: 0.1 µg/mL has been reported to immunostain SynGAP in neuron cultures fixed with 4% paraformaldehyde, 4% sucrose and perme-abilized with 0.1% Triton X-100 (2).

Research Sub CategorySynapse & Synaptic Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Silent synapses, or excitatory synapses that lack functional -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), are thought to be critical for regulation of neuronal circuits and synaptic plasticity. SynGAP, an excitatory synapse-specific RasGAP, regulates AMPAR trafficking, silent synapse number, and excitatory synaptic transmission in hippocampal and cortical cultured neurons. Over expression of SynGAP in neurons results in a remarkable depression of AMPAR-mediated miniature excitatory postsynaptic currents, a significant reduction in synaptic AMPAR surface expression, and a decrease in the insertion of AMPARs into the plasma membrane. Synaptic transmission is increased in neurons from SynGAP knockout mice as well as in neuronal cultures treated with SynGAP small interfering RNA.

Immunogen

peptide (KRLLDAQRGSFPPWVQQTRV) corresponding to amino acids 1274-1293 of rat SynGAP-a

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1071

Physical form

Protein A purified

Format: Purified

Purified in PBS with 0.05% NaN3 and 30% Glycerol

Specificity

SynGAP

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.