Analysis Note

ControlLysates from mouse liver, C2C12, Jurkat, and HUVEC

Application

Research CategorySignaling

Research Sub CategoryDevelopmental SignalingGPCR, cAMP/cGMP & Calcium Signaling

Anti-Frizzled 5 Antibody is an antibody against Frizzled 5 for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Wnt family consists of ligands involved in cell fate determination, organogenesis, and oncogenesis. The Wnt signal is thought to be transduced sequentially via a homolog of the frizzled family of receptors to GSK-3 (disheveled), β- and γ- catenin and finally to members of the Tcf/Lef-1 family of HMG box transcription factors. Frizzled 5 is believed to function as the receptor for the Wnt5a ligand.

Immunogen

Epitope: C-Terminus

KLH-conjugated, synthetic peptide derived from amino acids 564-585 of human Frizzled 5 (CTGRTPPGPAATYHKQVSLSHV).

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A chromatography

Purified antibody in PBS with 0.05% sodium azide and 30% Glycerol

Format: Purified

Quality

Routinely evaluated by immunoblot on lysates from mouse liver, C2C12, Jurkat or HUVEC cells

Specificity

Frizzled 5 (aka Fz5 or Fzd5)

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

Frizzled 5 (62kDa)