Analysis Note

ControlA431 cells, HEK293, Jurkat, MCF7 cell lysate.

Application

Research CategorySignaling

Anti-erbB-2/HER-2 Antibody is an antibody against erbB-2/HER-2 for use in IH, IP & WB.

Immunoprecipitation:
4 µg of a previous lot immunoprecipitated erbB-2/HER-2 from 600 µg MCF-7 RIPA lysate, and previously in A431, LNCaP, and c-erbB-2 transfected 3T3/NIH RIPA lysate.

Immunohistochemistry:
A previous lot of antibody has been reported by an independent laboratory, to detect erbB-2/HER-2 in frozen and formalin/paraffin sections of rat breast.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.

Immunogen

A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-1127

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified

Purified rabbit polyclonal IgG in buffer containing 10 mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Frozen solution.

Format: Purified

Quality

Routinely evaluated by Western Blot on HEK293 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Her-2 on 10 µg of HEK293 lysates.

Specificity

Recognizes c-erbB-2 (185 kDa), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

185 kDa