Analysis Note

ControlHuman tonsil tissue lysate

Application

Western Blot (Snap i.d.) Analysis: 5 µg/mL from a previous lot detected IL-18 in 10 µg of human tonsil tissue lysate.

Immunohistochemistry Analysis: A 1:600 dilution from a previous lot detected IL-18 in normal human spleen tissue.

Research Sub CategoryCytokines & Cytokine Receptors

Research CategoryInflammation & Immunology

This Anti-Interleukin-18 (IL-18) Antibody is validated for use in WB, IH(P) for the detection of Interleukin-18 (IL-18).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IL-18 has been shown to be a pro-inflammatory cytokine that induces interferon-gamma. IL-18 has also been found to have the ability to activate nuclear factor-kappaB (NFkB), express Fas ligand, induce CC and CXC chemokines, and also has been found to increase the production of viable human immunodeficiency virus. IL-18 has been linked to syndromes displaying symptoms of chronic low-levels of inflammation. IL-18 also seems to have some control over homeostasis and behavior in the central nervous system as well as neuroinflammatory activity.

Immunogen

KLH-conjugated linear peptide corresponding to human Interleukin-18.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human tonsil tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected IL-18 in 10 µg of human tonsil tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~22 kDa observed