Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Axin-1 in A431 and HeLa cells.

Immunohistochemistry Analysis: 1:1,000 dilution from a representative lot detected Axin-1 in human kidney and human skin tissues.

Anti-Axin-1 Antibody is an antibody against Axin-1 for use in WB, IC, IH.

General description

Axin-1 is a beta-catenin destruction complex scaffold protein and a transcription factor of the Wnt signaling pathway. Variation in the Wnt signaling pathway has been associated with the formation of some primary human cancers. Axin-1 mutation has been linked to oral squamous cell carcinoma. Axin-1 binds to GSK3alpha and activates the Wnt signaling pathway which is shown to be associated with growth and development of cells. Axin-1 has also been implicated in Alzheimer′s disease.

Immunogen

KLH-conjugated linear peptide corresponding to human Axin-1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 on 10 µg of HEK293 cell lysate.

Target description

~110 kDa observed. An uncharacterized band appears at ~40 and 45 kDa in some lysates.