Analysis Note

ControlHuman Alzheimer′s brain tissue.

Application

Immunohistohemistry Analysis: A 1:500 dilution from a representative lot detected Amyloid Beta (ABeta) x-42 in human pons/midbrain tissue.

ELISA Analysis: A representative lot detected Amyloid Beta (ABeta) x-42, but demonstrates a loss of signal against Amyloid Beta (ABeta) x-42.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Amyloid Beta (ABeta) x-42, but demonstrated a loss of signal against x-40 and not x-43 in WB (Prof. J. Buxbaum, Mt. Sinai School of Medicine).

Anti-Amyloid Beta (ABeta) x-42, clone 12F4 is an antibody targeting the Amyloid Beta (ABeta) x-42 protein, validated for use in IHC & ELISA.

General description

One of the most important and initial steps which causes loss of memory and cognition in Alzheimer's Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (Amyloid Beta) 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition.

Immunogen

KLH-conjugated linear peptide corresponding to human Amyloid Beta (ABeta) x-42.

Linkage

Replaces: 05-831

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Evaluated by Immunohistochemistry in human Alzheimer′s disease brain tissue.

Immunohistochemistry Analysis: A 1:2,000 dilution of this antibody detected Amyloid Beta (Abeta) x-42 in human Alzheimer′s disease brain tissue.

Specificity

This antibody detects Amyloid Beta (ABeta) x-42, but does not cross react with Amyloid Beta (ABeta) x-40 or Amyloid Beta (Abeta) x-43.

Target description

4 kDa calculated