Analysis Note

ControlLysates from rat brain cytosol fraction.

Application

Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect Tau (3-repeat isoform RD3) in autoclaved paraffin brain sections (de Silva, R., 2003).

Differential Detection of Tauopathies:
(Togo, T., 2002.)

Research Sub CategoryNeurotransmitters & ReceptorsNeurodegenerative Diseases

Research CategoryNeuroscience

Anti-Tau (3-repeat isoform RD3) Antibody, clone 8E6/C11 is an antibody against Tau (3-repeat isoform RD3) for use in IH & WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Microtubule Associated Proteins, or MAPS, bind to the tubulin subunits of microtubule structures and regulate their functional stability. In the cell MAPs bind to monomer and multimerized tubulin. MAP binding to multimerized tubulin further stabilizes the formation of higher order microtubulin structures. MAP binding to microtubule structures is mediated through phosphorylation through Microtubule Affinity Regulated Kinase (MARK). Phosphorylation releases MAPs bound to microtubules, destabilizing the structure, driving it toward disassembly. There are predominately two MAP types, I, II. Type II MAP includes MAP2, MAP4, and tau and are found in nervous tissue. Six tau isoforms exist in brain tissue, and they are distinguished by their number of binding domains. Three isoforms have three binding domains and the other three have four binding domains. The binding domains are located in the carboxy-terminus of the protein and are positively-charged (allowing it to bind to the negatively-charged microtubule). The isoforms with four binding domains are better at stabilizing microtubules than those with three binding domains.

Immunogen

Bovine thyroglobulin conjugated synthetic peptide corresponding to amino acids 209-224 (KHQPGGGKVQIVYKPV) of human Tau (isoform RD3). In other isoforms of huTau this sequence spans amino acids 267-316, omitting the second repeat domain where it bridges RD1 and RD3. The immunizing sequence is identical in human, mouse and bovine.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Mouse culture supernatant containing 0.05% sodium azide. Frozen at -20°C.

Unpurified

Quality

Routinely evaluated by western blot on rat brain cytosol fraction.

Western Blot Analysis:
A 1:1000-1:4000 dilution of this lot detected Tau (3-repeat isoform RD3) in lysates from rat brain cytosol fraction.

Specificity

Cross-reactivity with mouse and bovine expected due to sequence homology.

Recognizes Tau (3-repeat isoform RD3), Mr 45-65 kDa. Higher MW bands (68-72 kDa) represent phosphorylated Tau.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Target description

~45-65 kDa; phosphorylated forms at 68-72 kDa