Application

Research CategoryProtein Trafficking

Anti-phospho-HSP27 (Ser78) Antibody, clone JBW502 is an antibody against phospho-HSP27 (Ser78) for use in WB.

Research Sub CategoryChaperones

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

peptide corresponding to amino acids 73-83 of human HSP27 with a C-terminus cysteine added for conjugation (YSRAL[pS]RQLSS-C)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: 04-447

Physical form

Protein G Chromatography

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%

Format: Purified

Quality

routinely evaluated by immunoblot on phosphorylated HSP27 in 20mg of a cell lysate prepared from MCF-7 cells that were heat shocked for 30 minutes at 42°C, cultured for an additional 16 hours at 37°C, and then stimulated with 200mM arsenite for 30 minutes

Specificity

phosphorylated HSP27 (Ser78)

Storage and Stability

2 years at -20°C

Target description

27kDa