Analysis Note

ControlUV-treated 293 cell extracts, UV-treated HeLa cell extracts or breast cancer tissue

Application

Anti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho-Histone H2A.X (Ser139) also known as H2AXS139p.

Research Sub CategoryHistones

Additional Referenced Applications:
Immunohistochemistry Analysis: A representative lot detected Histone H2A.X (pSer139) in RNF168-WT and RNF 168-SA/SEKI mice lung tissue sections (Paraffin). (Xe, X., et al. (2015) Nat. Cell Biol. 20 (3); 320-331).
Chromatin Immunoprecipitation, see Meier, Andreas, et al. EMBO J., 26: 2707-18 (2007) in technical information tab.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

Immunogen

peptide (C-KATQA[pS]QEY) corresponding to amino acids 134-142 of human histone H2A.X

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MABE205

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Immunoaffinity Purified immunoglobulin in 0.1M Tris-Glycine,pH 7.4, 0.15M NaCl, 0.05% sodium azide as a preservative.

Quality

Immunoblot Analysis: 0.05-1 µg/ml of this antibody detected phosphorylated histone H2A.X (Ser139) in acid extracted histone lysates from Jurkat cells treated with 0.5 µM staurosporine (Catalog # 19-123).
Immunocytochemistry: 2 µg/ml of this antibody detected phosphorylated histone H2A.X in HeLa cells treated with 0.5 µM staurosporine for 4-6 hours.

Specificity

Recognizes Histone H2A.X phosphorylated at Ser139.

Storage and Stability

Maintain for 1 year at 2 to 8°C from date of shipment. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Target description

17 kDa