Application

Research CategoryApoptosis & Cancer

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Akt/PKB in A431 cells.
The unconjugated antibody (Cat. No. 05-591) is shown to be suitable also for flow cytometry, immunocytochemistry, immunoprecipitation, and Western blotting applications.

This Anti-Akt Antibody, PH Domain, clone SKB1, Alexa Fluor^® 647 is validated for use in Immunocytochemistry for the detection of PKB.

Research Sub CategoryKinases & Phosphatases

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

RAC-alpha serine/threonine-protein kinase (EC 2.7.11.1; UniProt P31749; also known as PKB, PKB alpha, Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha) is encoded by the AKT1 (also known as PKB, RAC) gene (Gene ID 207) in human. AKTs (protein kinase Bs) are serine/threonine kinases that play an important role in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. There exit three types of AKTs (α, β, γ or AKT 1, 2, 3) sharing a high degree of homology, but differing slightly in the localization of their regulatory phosphorylation sites. AKT α is the predominant isoform in most tissues, whereas the highest expression of AKT β is observed in the insulin-responsive tissues, and AKT γ is abundant in brain tissue. Each AKT type is composed of three functionally distinct regions, including an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif.

Immunogen

GST-fusion protein corresponding to residues 1-149 of human Akt1.

Epitope: Amino acids 1-149

Legal Information

ALEXA FLUOR is a registered trademark of Life Technologies

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Protein G purified.

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Akt/PKB in HeLa cells.

Specificity

Clone SKB1 recognizes Akt1 and Akt2, but not Akt3, GST fusion proteins at 100 ng by immunoblotting analysis using the unconjugated antibody (Cat. No. 05-591).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed.