Application

Anti-Nucleolin Antibody, clone 3G4B2 is a high quality Mouse Monoclonal Antibody for the detection of Nucleolin & has been validated in IP, WB, ICC.

General description

Nucleolin, also known as C23, is the major nucleolar protein of exponentially growing eukaryotic cells. It is a ubiquitous conserved multi-domain phosphoprotein found in all vertebrate species. Nucleolin contains a specific bipartite nuclear localization signal sequence and three different structural domains. Nucleolin is a multifunctional protein involved in most steps of ribosome biogenesis. Nucleolin has been implicated in chromatin structure, rDNA transcription, rRNA maturation, ribosome assembly, cytokinesis, nucleogenesis, cell proliferation and growth, and
nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.

Immunogen

Nuclear matrix extract from MDCK cells

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MABE466

Physical form

mouse culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%

Quality

routinely evaluated by immunoblot on HeLa nuclear extract

Specificity

nucleolin

Target description

100kDa