Analysis Note

ControlHuman normal pancreas tissue

Application

Research CategorySignaling

Research Sub CategoryInsulin/Energy Signaling

This Anti-Pro-Insulin C-Peptide Antibody, clone C-PEP-01 is validated for use in IH(P) for the detection of Pro-Insulin C-Peptide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pro-Insulin consists of the three parts: C-peptide and two long strands of amino acids (alpha and beta chains; later become linked together to form the Insulin molecule). From every molecule of Pro-Insulin, one molecule of Insulin plus one molecule of C-peptide are produced. C-peptide is released into the blood stream in equal amounts to Insulin.

Immunogen

Synthetic C-peptide of human pro-insulin conjugated to bovine serum albumin.

Linkage

Replaces: CBL94

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Sequential precipitation with caprylic acid and ammonium sulfate.

Mouse monoclonal IgG1 in phosphate buffered saline, pH7.4, and 15 mM sodium azide.

Quality

Evaluated by Immunohistochemistry in normal human pancreas.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected insulin in human normal pancreas tissue.

Specificity

No cross-reactivity with Insulin or other peptide hormones or proteins was observed.

The antibody C-PEP-01 reacts specifically with C-peptide, a part of the Pro-insulin molecule.

Storage and Stability

Store at 2-8°C and use within 1 year from date of receipt.

Target description

Approx. 12 kDa