Analysis Note

ControlHuman placenta tissue lysate

Application

Research Sub CategoryImmunoglobulins & Immunology

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC (Osamu, N., et al., 2008).

Immunochistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Osamu, N., et al., 2008).

Detect B7-H3 (CD276) using this Anti-B7-H3 (CD276) Antibody, clone MJ8 validated for use in WB, FC, IH.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

B7 homolog 3 (B7-H3 or CD276) is a costimulatory molecule belonging to the B7 family that signals through the CD28 family of molecules, including CD28, CTLA4 and ICOS. B7-H3 may be involved in the regulation of T-cell-mediated immune response and may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis, as well as a role of marker for detection of neuroblastoma cells. B7-H3 may also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. B7-H3 is ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes, and it is weakly expressed in resting monocytes. B7-H3 is expressed in dendritic cells derived from monocytes, epithelial cells of sinonasal tissue, extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

Immunogen

Epitope: Extracellular Domain

Mouse B7-H3 Ig2a FC fusion protein, consisting of the extracellular domain linked to the Fc portion of mouse IgG2a.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Format: Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human placenta tissue lysate.

Western Blot Analysis: 0.25 µg/mL of this antibody detected BN-H3 on 10 µg of human placenta tissue lysate.

Specificity

This antibody recognizes the extracellular domain of B7-H3 (CD276).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~31 kDa observed molecular weight on human lysates corresponds to isoform 2 of B7-H3.