HiTrap IMAC HP is prepacked with IMAC Sepharose High Performance. The resin is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent high-resolution purification of polyhistidine tagged proteins.

• For optimizing purification of histidine-tagged proteins when Ni²⁺ is not the best choice of metal ion
• Conveniently charge with your metal of choice
• Small bead size for high-performance purification
• High binding capacity

IMAC Sepharose HP is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu²⁺, Zn²⁺, Ni²⁺, or Co²⁺); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose.