Application

Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected UBR5 in HeLa cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected UBR5 in NIH3T3, PC12 and HepG2 cell lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected UBR5 in HeLa, A431, and NIH 3T3 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected UBR5 in human testis tissue sections.Affinity Binding Assay: A representative lot of this antibody bound recombinant UBR5 protein fragment with a KD of 1.2 x 10-6 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-UBR5, clone 2F14 ZooMAb, Cat. No. ZRB1698, is a recombinant Rabbit monoclonal antibody that specifically targets URB5 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

His-tagged recombinant fragment corresponding to 231 amino acids from the C-terminal half of human E3 ubiquitin-protein ligase UBR5.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 2F14 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects E3 ubiquitin-protein ligase UBR5. It targets an epitope within the C-terminal half.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

E3 ubiquitin-protein ligase UBR5 (UniProt: O95071; also known as EC:2.3.2.26, E3 ubiquitin-protein ligase, HECT domain-containing 1, HECT-type E3 ubiquitin transferase, UBR5, Hyperplastic discs protein homolog, hHYD, Progestin-induced protein) is encoded by the URB5 (also known as EDD, EDD1, HYD, KIAA0896) gene (Gene ID: 51366) in human. UBR5 is a widely expressed nuclear protein that is shown to be most abundant in testis. It is also expressed at high levels in brain, pituitary, and kidney. URB5 is identified as a novel regulator of p53 and downregulation of UBR5 results in elevated levels of p53 in normal and transformed cells. Its deficiency in mice is reported to result in mid gestation embryonic death due to a compromised yolk sac vascular development. UBR5 is a component of the N-end rule pathway. It recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. It is shown to be involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. It also plays a role in the control of cell cycle progression. It associates with CDK9 and TFIIS/TCEA1 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5, and TFIIS/TCEA1 that can stimulate target gene transcription. UBR5 regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response and may have a role in tumor suppression. Two isoforms of UBR5 are described that are produced by alternative splicing and differ only in a single amino acid. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Smits., VAJ. (2012). Cell Cycle 11(4); 715-720).