Anti-ZNF638, clone 4C19 ZooMAb� Rabb

Application

Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected ZNF638 in NIH3T3 cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected ZNF638 in PC12 cell lysate.Affinity Binding Assay: A representative lot of this antibody bound ZNF638 peptide with a KD of 4.1 x 10-7 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ZNF638 in human colon tissue sections.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ZNF638 in HeLa, A431, HepG2, and NIH 3T3 cells.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-ZNF638, clone 4C19 ZooMAb, Cat. No. ZRB1186, is a recombinant Rabbit monoclonal antibody that targets ZNF638 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human Zinc Finger Protein 638 (ZNF638).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 4C19 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Zinc Finger Protein 638 (ZNF638). It targets an epitope within 21 amino acids from the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Zinc finger protein 638 (UniProt: Q14966; also known as Cutaneous T-cell lymphoma-associated antigen se33-1, CTCL-associated antigen se33-1, Nuclear protein 220, Zinc finger matrin-like protein, ZNF638) is encoded by the ZNF638 (also known as NP220, ZFML) gene (Gene ID: 27332) in human. ZNF638 is a nucleocytoplasmic transcription factor that binds to cytidine clusters in double-stranded DNA and plays a key role in the silencing of unintegrated retroviral DNA. It is also shown to act as an early regulator of adipogenesis by acting as a transcription cofactor of CEBPs, controlling the expression of PPAR and other pro-adipogenic genes, such as SREBF1. ZNF638 is selectively expressed in mature thermogenic adipocytes and tissues and its induction in response to classic stimuli promotes heat generation. It is shown to be upregulated by cAMP modulators in vitro and by cold exposure and by pharmacological stimulation of β-adrenergic signaling in vivo. Ectopic expression of ZNF638 is reported to increase adipogenesis in vitro, whereas its knockdown inhibits differentiation and decreases the expression of adipocyte-specific genes. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Perie, L., et al. (2019). J. Endocrine Soc. 3(12); 2326-2340; Meruvu, S., et al. (2011). J. Biol. Chem. 286(30); 26516-26523).