Application
Anti-Bmi-1, clone 1G8 ZooMAb, Cat. No. ZRB1155, is a recombinant Rabbit monoclonal antibody that targets Bmi-1 and has been tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected Bmi-1 in MCF-7 cell lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Bmi-1 in K562 and Cos-7 cell lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in HeLa, A431, HepG2, and NIH 3T3 cells.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Bmi-1 in human kidney tissue sections.Affinity Binding Assay: A representative lot of this antibody bound Bmi-1 peptide with a KD of 2.5 x 10-7 in an affinity binding assay. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Bmi-1.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Specificity
Clone 1G8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Bmi-1. It targets an epitope within 19 amino acids from the C-terminal region.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Target description
Polycomb complex protein BMI-1 (UniProt: P35226; also known as Polycomb group RING finger protein 4, RING finger protein 51) is encoded by the BMI1 (also known as PCGF4, RNF51) gene (Gene ID: 100532731) in human. BMI-I (B cell-specific Moloney murine leukemia virus integration site 1) is a component of the polycomb repressive complex 1 (PRC1) that is required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. This complex acts via chromatin remodeling and modification of histones and mediates mono-ubiquitination of histone H2A lysine 119, rendering chromatin. The canonical PRC1 complex consists of four core subunits: CBX (polycomb; CBX2/4/6/7/8), PCGF (polycomb group factors; PCGF1 6), PHC (polyhomeotic homologues; PHC1/2/3), and RING E3 ligase (RING1A/B). BMI-I has three domains. The N-terminal RING domain, a central domain, and a C-terminal proline-rich domain. The RING domain forms a complex with RINGA/B proteins that constitutes the heterodimeric E3 ubiquitin ligase subunit of the PRC1 complex. The central is a ubiquitin-like (UBL) domain and is involved in protein-protein interactions and its binding partners include the polyhomeotic proteins (PHC1, PHC2, PHC3). The central domain of BMI-I is reported to be essential for its oncogenic activity and is shown to be essential for transcriptional repression activity. Higher expression of BMI1 gene is overexpressed in certain mantle cell lymphomas and several other cancers. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Gray, F., et al. (2016). Nat. Commun. 7; 13343).