Anti-LC3A, clone 3J12 ZooMAb� Rb mono

Application

Quality Control TestingWestern Blotting Analysis: A 1:10,000 dilution of this antibody detected LC3A in Rat Brain tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected LC3A in Human brain, mouse brain and PC12 lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LC3A in SH-SY5Y cells.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LC3A in human cerebral cortex tissue sections.Affinity Binding Assay: A representative lot of this antibody bound LC3A peptide with a KD of 3.3 x 10-8 in an affinity binding assay.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-LC3A, clone 3J12 ZooMAb, Cat. No. ZRB1125, is a recombinant Rabbit monoclonal antibody that specifically targetsLC3A and has been tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of human LC3A.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 3J12 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Autophagy-related protein LC3A. It targets an epitope within 25 amino acids from the N-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Microtubule-associated proteins 1A/1B light chain 3A (UniProt: Q9H492; also known as Autophagy-related protein LC3A, Autophagy-related ubiquitin-like modifier LC3A, MAP1 light chain 3-like protein 1, MAP1A/MAP1B light chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated protein 1 light chain 3 alpha) is encoded by the MAP1LC3A gene (Gene ID: 84557) in human. LC3s are structural proteins of autophagosomal membranes and are widely used as biomarkers of autophagy. They are ubiquitously distributed in all eukaryotes and are expressed as three splice variants known as LC3A, LC3B, and LC3C. LC3A is mainly localized to perinuclear and nuclear regions, LC3B is evenly distributed throughout the cytoplasm and is also localized in the nucleolar regions, and LC3C is shown to be located in the cytoplasm and strongly in the nuclei. Two variants (v1 and v2) of LC3A have been described that have different transcriptional start sites. Nuclear LC3A is shown to co-localize with Beclin-1. All three LC3s show a conserved region that is required for autophagosome formation. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Koukourakis, MI., et al. (2015). PLoS ONE 10(9); e0137675).