Application

Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected Stathmin-2 in Neuro-2A lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Stathmin-2 in PC12 and SH-SY5Y lysates.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Stathmin-2 in SH-SY5Y cells. Affinity Binding Assay: A representative lot of this antibody bound Stathmin-2 peptide with a KD of 1.5 x 10-8 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Stathmin-2 in human cerebral cortex tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Stathmin-2, clone 1B3 ZooMAb, Cat. No. ZRB1073, is a recombinant Rabbit monoclonal antibody that specifically targets Stathmin-2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Stathmin-2.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

30 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 1B3 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Stathmin-2. It targets an epitope within 20 amino acids from the N-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Stathmin-2 (UniProt: Q93045; also known as Superior cervical ganglion-10 protein, Protein SCG10) is encoded by the STMN2 (also known as SCG10, SCGN10) gene (Gene ID: 11075) in human. Stathmin-2 is a neurophosphoprotein that acts as a regulator of microtubule stability. It is a peripheral membrane protein that is also found in Golgi and endosomes. Its membrane attachment domain is localized in amino acids 1-26 and phosphorylation/regulatory domain is in amino acids 39-96. When phosphorylated by MAPK8, it stabilizes microtubules and consequently controls neurite length in cortical neurons. Its N-terminal palmitoylation promotes specific anchoring to the cytosolic leaflet of Golgi membranes and subsequent vesicular trafficking along dendrites and axons. In the developing brain, it is reported to negatively regulate the rate of exit from multipolar stage and retards radial migration from the ventricular zone. Stathmin-2 is considered as novel regulator of glucagon secretion and its expression has been reported in murine pancreatic alpha cells where its overexpression increases localization of glucagon into the endosomal-lysosomal compartment and depletion results in reduced endosomal localization of glucagon. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Asadi, F., and Dhanvantari, S. (2020). Front Endocrinol.11; 29).