Anti-Nectin-2/CD112,cl2H6ZooMAb�Rbmono

Application

Anti-Nectin-2/CD112, clone 2H6 ZooMAb, Cat. No. ZRB1029, is a Rabbit monoclonal antibody that specifically targets Nectin-2/CD112 and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Quality Control TestingWestern Blotting Analysis: A 1:1,000 dilution of this antibody detected Nectin-2/CD112 in MCF-7 cell lysate.Tested applicationsImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Nectin-2 in MCF-7 cells.Affinity Binding Assay: A representative lot of this antibody bound Nectin-2 peptide with a KD of 3.9 x 10-9 in an affinity binding assay.Flow Cytometry Analysis: 1 μg from a representative lot detected Nectin-2 in one million MCF-7 cells.Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Nectin-2 in human testis tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal extracellular domain of human Nectin-2.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 2H6 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Nectin-2. It targets an epitope within 24 amino acids from the N-terminal, extracellular domain.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Nectin-2 (UniProt: Q92692; also known as Herpes virus entry mediator B, Herpesvirus entry mediator B, HveB, Nectin cell adhesion molecule 2, Poliovirus receptor-related protein 2, CD112) is encoded by the NECTIN2 (also known as HVEB, PRR2, PVRL2) gene (Gene ID: 5819) in human. Nectin-2 is a single-pass type I membrane protein that displays ubiquitous expression. It is a Ca2+-independent cell-cell adhesion molecule that regulates cell adhesion between epithelial cells through the formation of transdimers between adjacent cells. Nectin-2-mediated cell adhesion is shown to induce the formation of E-cadherin-based adherens junctions followed by the formation of claudin based tight junctions. Nectin-2 also serves as a modulator of T-cell signaling and it can be either a co-stimulator of T-cell function, or a co-inhibitor depending on the receptor it binds to. Upon binding to CD226, it stimulates T-cell proliferation and cytokine production, including that of IL-2, IL-5, IL10, IL-13, and Interferon-g. Its interaction with PVRIG, inhibits T-cell proliferation. These interactions are shown to be competitive in nature. Over-expression of Nectin-2 has been reported in breast and ovarian cancer tissues and in several cancer cell lines. It is synthesized with a signal peptide (aa 1-31), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 32-360), a transmembrane domain (aa 361-381), and a cytoplasmic domain (aa 382-538). Nectin-2 has one Ig-Like V-type domain (aa 32-156) and two Ig-like C2 type domains (aa 162-256 and 261-345). This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Oshima, T., et al. (2013). Mol. Cancer. 12; 60).