Application

TruQuant IQQ is a high-quality quantitation system for making simultaneous accurate biological measurements on several hundred biochemicals in small quantities of biological samples. This is achieved by (1) spiking a complex Internal Standard (WORKFLOW-A) into a biological sample to a) quantify all the biochemicals in the sample relative to their counterparts in the Internal Standard, b) suppression-correct each compound and c) normalize sample to sample variances; and (2) injecting the same well characterized Long-Term Reference Standard (WORKFLOW-B) to create a daily retention time (RT) library of all compounds to be found in the Internal Standard for reproducible ID, and to measure day-to-day (QA/QC) to assure reproducible instrument performance. The system is completely automated using IROA ClusterFinder^™ software.IROA TruQuant IQQ Workflow Kit contains the materials and tools for the analysis of 90 experimental samples. The kit is intended to be used for mass spectrometry metabolomics applications.

Features and Benefits

Identification: Confidence in identifying common and new metabolitesQuantification: Comprehensive correction of analytical and biological variability for all analytesQuality Control: Convenient and automated tools for assuring reproducibility within/across studies Simultaneous metabolite identification, quantitation and platform QC Accurate compound formula ID for MS alone; complete ID with addition of SWATH, or IM IROA patterns discriminate peaks of biological origin from artefactual peaks allowing the removal of false data IROA-based fragments have the IROA ratio pattern of their parent peaks and can be identified as such using the “peak correlation” ClusterFinder^™ module Retention Index within the sample allows “unknowns” to be tracked with precision Suppression-corrected measurements for significantly better quantitation Normalizes (suppression-corrected) experimental samples to overcome day-to-day, instrument-to-instrument variances ClusterFinder^™ software solution builds libraries, IDs/quantitates compounds and normalizes data Ensures high level QC for accurate and reproducible results

Legal Information

ClusterFinder is a trademark of IROA Technologies LLC

Other Notes

ClusterFinder^™ software package is distributed with and is tailored to work with the IROA TruQuant IQQ kit. A user manual and video instructions are provided.To download the software package, click here - ClusterFinder software.Link to the ClusterFinder^™ Videos below: IROA Peaks Basics CF Process Overview CF Initiation CF New Project & Add Data CF Unbiased Search for IROA peaks CF Preferences CF Libraries & Methods CF BinTree - bins are displayed as a tree CF Curation 1: Untargeted - quality of the peaks CF Curation 2: CascadeTo access the MS Calculator Cascade Spreadsheet, follow the link below:MS Calculator