MLK4; ACTIVE

Application

Useful for kinase assay and Western blot.

Biochem/physiol Actions

Recombinant human MLK4 (114-420) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. MLK4 or mixed lineage kinase 4 belongs to the superfamily of MAP kinase kinase kinases (MAP3K1) which contain both ser/thr and tyr kinases activity in their catalytic domains. The structure of this kinase family incorporates an N-terminal Src homology (SH3) domain, followed by the kinase domain, a leucine zipper region, and a CDC42 /RAC -interactive binding (CRIB) motif, but divergent C-terminal regions. MLK4 is highly expressed in kidney and pancreas ⁽¹⁾. MLK4 is a negative regulator of TLR4 ⁽²⁾.

Legal Information

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Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.