SBK1; ACTIVE

Application

Useful for kinase assay.

Biochem/physiol Actions

Full-length recombinant human SBK1 was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. SBKI or SH3 domain binding kinase 1 is a novel serine/threonine protein kinase structurally related to a Xenopus gastrula-specific protein kinase, Pk9.7 which possessing a consensus sequence for an SH3-binding domain from developing rat brain and it play an important role in signal-transduction pathways related to the control of brain development ⁽¹⁾. SBK1 may predict a broad cellular function, and its dysregulated in certain cancers suggests an involvement of the protein in the pathogenesis of human cancers ⁽²⁾.

Legal Information

PRECISIO is a registered trademark of Sigma-Aldrich Co. LLC

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.