Biochem/physiol Actions

ST045849 (TT04) is an O-GlcNAc transferase (OGT) Inhibitor (IC₅₀/human splice variant = 30 µM/sOGT & 53 µM/ncOGT; 6.25 µM UDP-GlcNAc, 500 µM peptide substrate KKKYPGGSTPVSSANMM). ST045849 is typically employed in the 1-100 µM range for probing O-GlcNAcylation-dependent cellular processes in cultures, although low 10-50 nM and high 1-20 mM dosing ranges have also been reported. Daily oral administration is reported to prevent embryos neural tube defects at E10.5 among diabetic pregnant mice (20 mg/kg/day p.o. from E6.5 to E9.5).