DFHBI-1T

Biochem/physiol Actions

DFHBI-IT is a membrane-permeable, non-cytotoxic, RNA aptamers-activated fluorescence probe widely employed in varous detection systems utilizing compatible RNA tags such as Spinach, Spinach2, iSpinach, and Broccoli. Upon binding Spinach2, DFHBI-1T offers brighter fluorescence than DFHBI due to increased extinction coefficient & quantum yield (by 1.4- and 1.3-fold, respectively). DFHBI-1T is also better suited for devices optimized for GFP or fluorescein detection/imaging due to its red shift in excitation & emission wavelengths (Spinach2-comlex Ex/Em = 482/505 nm with DFHBI-1T vs 447/501 nm with DFHBI).