Application

ABScript II One Step RT-qPCR Probe Kit is designed for use in real-time RT-PCR (or RT-qPCR) applications, including detection of RNA in samples being analyzed.For Research Use Only.Application NoteAdditional Materials Required but Not Included: RNA templates, primers and probes Optical-grade qPCR tubes, plates, sealing films, and aerosol-resistant pipette tipsCompatible Instruments:*50X ROX Reference Dye IApplied Biosystems 7000/7300/7700/7900, Applied Biosystems StepOne^™/StepOnePlus^™**50X ROX Reference Dye IIApplied Biosystems 7500/ViiA^™7, QuantStudio, Stratagene Real-time PCR Systems, Rotor-gene^® 3000NO ROX Reference DyeBio-Rad iCyclers/ CFX96^™/ CFX384^™, Roche^® Light Cyclers, QIAGEN/Corbett Systems, Eppendorf Mastercyclers^®.

Components

2X One Step RT-qPCR Probe Buffer* One Step Probe HS Taq ABScript II RT Enzyme Mix50X ROX Dye I *50X ROX Dye II **RNase-free ddH2O See datasheet for more information.Reaction SizeReaction size is based on a 50 uL reaction volume.The 2x One Step RT-qPCR Probe Buffer is supplied in the following volumes:20RXN: 0.5 mL100RXN: 2.5 mL500RXN: 1.25 mL

Features and Benefits

Ready-to-use one-step RT-qPCR probe kit with all components for RT-qPCR (except primers, probes and template RNA) Uses the robust ABScript II Reverse Transcriptase (RT), which provides reliable and efficient reverse transcription on a wide range of RNA template amount This one-step kit improves sensitivity and effectively prevents contamination Flexible format, as the ABScript II One Step RT-qPCR Probe Kit includes two separate 50X concentrations of the ROX (passive reference) dye, to be added as required by specific qPCR instruments.

General description

The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. The one-step format prevents contamination and allows for higher sensitivity. This kit is ideal for analyzing low input RNA samples.The ABScript II Reverse Transcriptase in the kit provides reliable reverse transcription of RNA. After reverse transcription, the hot-start version of Taq polymerase is activated at 95°C and the ABScript II Reverse Transcriptase is inactivated simultaneously. In the sequential PCR reaction, the 5′-3′ exonuclease activity of Taq polymerase cleaves the hybridized probe, separating the reporter from the quencher and releasing fluorescent signal.

Legal Information

StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Mastercycler is a registered trademark of Eppendorf AG

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

Eppendorf is a registered trademark of Eppendorf AG

ViiA is a trademark of ThermoFisher

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Rotor-Gene is a registered trademark of Qiagen GmbH

Roche is a registered trademark of Roche Molecular Systems, Inc.

QuantStudio is a trademark of IROA Technologies LLC

StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries