Application

Sheepshead minnow (Cyprinodon variegatus), 7-day Chronic, daily renewal, SSW proficiency testing material is designed for use in chronic Whole Effluent Toxicity (WET) proficiency testing.Have any questions? Explore our FAQs.For more information on this product, please contact our PT Service Team.

Features and Benefits

We have a vast portfolio of environmental PT schemes with "fit-for-purpose" samples to help you meet the needs of the laboratory.Our proficiency testing (PT) program is a benchmark to: provide staff with an insight into their laboratory′s performance, to verify the accuracy and reliability of their testing process provide a comparison of performance with that of other laboratories, nationally and internationally test and educate staff, provide a better understanding of the impact of incorrect results and identify areas where there may be problems provide an independent evaluation of laboratory performance demonstrate to clients, colleagues and accreditation bodies that there is a commitment to quality

General description

This Whole Effluent Toxicity (WET) proficiency testing sample is produced in accordance with ISO/IEC 17043:2010. This sample is manufactured according to TNI requirements. However, please note, this sample may include non-TNI analytes as well.In the chronic WET tests, Sheepshead minnow isemployedas a test organismto measureits ability to grow, develop and reproduce when exposed to wastewatereffluents.This method of determination is used to estimate the effluent′s toxicity levelsin aquatic/freshwater environments.Supelco^® is a global proficiency testing (PT) provider that manufactures best-in-class environmental and pharmaceutical PT products. Most water PT samples are available as concentrates or ready-to-use volumes giving the end-user flexibility in selecting samples that will fit their exact needs. Our PT program is designed to meet the quality assurance requirements in compliance with Discharge Monitoring Report - Quality Assurance Program (DMR-QA), thereby ensuring the analytical competence of your laboratory.Analyte/s: Values of analytes vary lot to lot.All information regarding the use of Sheepshead minnow (Cyprinodon variegatus), 7-day Chronic, daily renewal, SSW proficiency testing standard can be found in the reporting packet which will be available to download at PT Portal.

Legal Information

Supelco is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Are you new to Supelco^® Proficiency TestingͰ Be sure to register your account details in the PT Portal before placing your order.This will provide access to data entry, reporting packets and final reports. Registrations will be approved shortly after your order is placed. For further questions, write to us at [email protected] placing your order online, please indicate your study preference under the ′your reference′ column. Available studies can be viewed at the bottom of the page.Click here to learn more about Quick-Turn Studies (on-demand proficiency tests that are offered depending on lot availability). The Quick-Turn results are released within 48-72 hours whereas scheduled study reports are released within 21 days.Proficiency testing samples are presented as single blind. No values for the samples are released until the testing period concludes.Whole Effluent Toxicity (WET) tests are designed to predict the impact and toxicity of effluents discharges.Proficiency Testing Schedule 2023DMR-QA (US customers only)DMR-QA 43 2023 Dates TBDQuick-Turn Study (on demand proficiency test that is offereddepending on lot availability)

Preparation Note

Description• The effluent is provided as a concentrate for dilution in synthetic seawater.• This concentrate is an EPA-specified toxicant in reagent-grade DI water.• An ampule is provided for making fresh effluents for each day of the test.• Do not deviate from the supplied instructions or EPA Method 1004.0. • Contact [email protected] before running the test if you see the need to deviate from the test conditions.• Deviations may affect your final evaluation.Preparation Instructions• To prepare 3 L of effluent, add 2900 mL of synthetic seawater to a volumetric flask. • Preparation instructions for synthetic seawater can be found in the EPA document EPA-821-R-02-014, page 26.• Transfer the entire contents of one container to the flask.• Rinse the container with ampule seawater and transfer to the volumetric flask. Bring flask to 3 L volume with synthetic seawater.• This represents 3 L of effluent water for the test.Required Test Condition• Test Type: Static renewal• Test duration: 7 days• Temperature: 25 ºC ±1 ºC. Test must not deviate by more than 3 ºC during the test.• Renewal of Test solutions: Daily• Dilution water: Synthetic seawater ,Test Conditions• Light quality: Ambient laboratory illumination (recommended)• Light intensity: 10-20 μE/m²/s (50-100 ft-c) (ambient laboratory levels) (recommended) • Photoperiod: 16 h light, 8 h darkness (recommended) • Test chamber size: 600 mL - 1 L beakers or equivalent (recommended) • Test solution volume: 500-750 mL/replicate (loading and DO restrictions must be met) (recommended) • Age of test organisms Newly hatched larvae (less than 24 h old; less than or equal to 24-h range in age) (required) • No. of larvae per test chamber: 10 (required minimum) • No. of replicate chambers per concentration 4 (required minimum) • No. of larvae per concentration: 40 (required minimum) • Source of food: Newly hatched Artemia nauplii, (less than 24-h old) (required) • Feeding regime: Feed once a day 0.10 g wet weight Artemia nauplii per replicate on Days 0-2; Feed 0.15 g wet weight Artemia nauplii per replicate on Days 3-6 (recommended)• Cleaning: Siphon daily, immediately before test solution renewal and feeding (required) • Aeration: None, unless DO falls below 4.0 mg/L, then aerate all chambers. The rate should be less than 100 bubbles/minimum (recommended) • Endpoints: Survival and growth (weight) (required)• Test acceptability criteria: 80% or greater survival in controls; average dry weight per surviving organism in control chambers must be 0.60 mg or greater, if unpreserved, or 0.50 mg or greater after no more than 7 days in 4% formalin or 70% ethanol (required)

Storage and Stability

Please refer to sample label for expiration date, specific storage temperature, and specific storage conditions. If storage conditions are not provided on the label, it is intended for room temperature storage.