Analysis Note

Positive ControlA431 cells, HeLa cells

Application

Immunoblotting (1:2500)Immunoprecipitation (40 l/500g total protein from lysate)Immunocytochemistry (1:500)

General description

This Anti-Rad51 (Ab-1) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of Rad51 (Ab-1).

Affinity purified rabbit polyclonal antibody. Recognizes the ~43 kDa Rad51 protein.

Recognizes the ~43 kDa Rad51 protein in ovarian tissue.

The bacterial RecA and the yeast RAD51 proteins are involved in homologous recombination and DNA repair. The human RAD51 protein is the homologue of these two proteins. While the modular structure of these proteins is conserved and the homology is very strong with the bacterial protein there are functional differences seen with RAD51 including an ATP hydrolysis rate 1/10 that seen with RecA. Although RAD51 is a nuclear protein and is largely absent from the cytoplasm and nucleoli, the protein lacks a nuclear localization signal and requires other proteins in a complex to be translocated into the nucleus. Treatment of cells with DNA synthesis inducing agents will cause an increase in the levels of RAD51 protein which then undergo a shift in distribution becoming focal in nature. This focal distribution may be blocked by inhibitors of transcription. RAD51 can be shown to interact directly with the chromosomes and during the process of meiosis, its distribution of RAD51 undergoes dynamic change. RAD51 has been shown to interact with a wide variety of different proteins that include the wild-type form of p53, BRCA2, and RAD52, which specifically modulates its activity.

Immunogen

recombinant, human Rad51

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Brendel, V., et al. 1997. J. Mol. Evol.44, 528.Boulikas, T., et al. 1997. Anticancer Res.17, 843.Gupta, R.C., et al. 1997. Proc. Natl. Acad. Sci. USA94, 463.Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.Sharan, S.K., et al. 1997. Nature386, 304.Li, M.J., et al. 1996. Proc. Natl. Acad. Sci. USA93, 10222.Shen, Z., et al. 1996. J. Biol. Chem.271, 148.Sturzbecher, H.W., et al. 1996. EMBO J. 15, 1992.Tashiro, S., et al. 1996. Oncogene12, 2165.Ashley, T., et al. 1995. Chromosoma104, 19.Haaf, T., et al. 1995. Proc. Natl. Acad. Sci. USA92, 2298.

Due to a high degree of sequence identity, this antibody is also expected to cross-react with bovine, equine, and canine RAD51. Antibody should be titrated for individual systems.

Packaging

100 µL in Plastic ampoule

Physical form

In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)