Application

The Engineered Control cells can be used for drug permeability assays. They may also be used as the control in conjunction with C2BBe1 Transporter Knockout cell lines for drug/transporter interaction studies. Engineered control cells have been used in the in vitro Caco-2 permeability assay. The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger NucleasesComparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 GenesCaco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human-derived, and grow in a homogenous monolayer that forms tight junctions which is necessary for efflux ratio analysis.

General description

The C2BBe1 cells, a sublcone of Caco-2 cells, correspond to ATCC CRL-2101. The C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years).

Legal Information

This product is covered under a consumable purchase agreement for one-time use only. For more information:ADME/Tox Cell Lines License

Quality

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.