Application

Glycerol-3-Phosphate Assay Kit has been used in the determination of glycerol-3-phosphate and phosphoenolpyruvate levels. It has also been used to quantify the concentration in aerial mycelium.

General description

Glycerol 3-phosphate (G3P) is an important intermediate of carbohydrate and lipid metabolic pathways. It is produced from glycerol by glycerol kinase or from dihydroxyacetone phosphate by glycerol 3-phosphate dehydrogenase. G3P may enter the G3P shuttle to generate NAD⁺, or may be converted to glyceraldehyde 3-phosphate and enter glycolysis or the lipid biosynthesis pathway.

Principle

The Glycerol 3-Phosphate Colorimetric Assay Kit provides a simple assay for measuring G3P in various tissues and cells (ranging from 2-10 nmole/well). G3P is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the amount of G3P present.

Suitability

Suitable for the detection of Glycerol-3-Phosphate in tissue and cell culture samples.