General description
Aldehydes are highly reactive molecules, which play a role in many physiological, pathological and commercial processes. They have been implicated as environmental pollutants, and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool for biological and chemical research, the food industry, and environmental pollution surveillance.The Fluorometric Aldehyde Assay Kit provides a simple and direct procedure for measuring aldehydes in samples in which the pH is physiological and higher. Aldehyde reacts with a fluorogenic dye, resulting in a fluorometric (λex = 365/λem = 435 nm) product proportional to the amount of aldehydes present. The Fluorometric Aldehyde Assay Kit has a lower limit of detection of 0.3 nanomole of aldehyde in a 100 µL assay volume (3 µM).
Aldehydes are highly reactive molecules, which play a role in many physiological, pathological and commercial processes. They have been implicated as environmental pollutants and in neurodegeneration and DNA damage. A method for rapid and accurate measurement of aldehydes is an important tool for biological and chemical research, the food industry and environmental pollution surveillance.
Principle
The fluorometric aldehyde assay kit provides a simple and direct procedure for measuring aldehydes in samples in which the pH is physiological and higher. Aldehyde reacts with a fluorogenic dye, resulting in a fluorometric (λex = 365/λem = 435 nm) product proportional to the amount of aldehydes present. The fluorometric aldehyde assay has a lower limit of detection of 0.3nanomole of aldehyde in a 100µL assay volume (3µM).
Suitability
This kit is suitable for the detection of aldehydes in a variety of samples, particularly when the samples are at a higher pH. When working with samples in a low pH, product number MAK140, Colorimetric Aldehyde Assay, is more suitable.
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