Application

Lipid peroxidation (MDA) assay kit has been used to determine the levels of malondialdehyde (MDA).

General description

Lipid peroxidation is the degradation of lipids that occurs as a result of oxidative damage and is a useful marker for oxidative stress. Polyunsaturated lipids are susceptible to an oxidative attack, typically by reactive oxygen species, resulting in a well-defined chain reaction with the production of end products such as malondialdehyde (MDA). Lipid peroxidation may contribute to the pathology of many diseases including atherosclerosis, diabetes, and Alzheimer′s.

Principle

In this kit, lipid peroxidation is determined by the reaction of MDA with thiobarbituric acid (TBA) to form a colorimetric (532 nm)/fluorometric (λ_ex = 532/λ_em = 553 nm) product, proportional to the MDA present.

Suitability

Suitable for the measurement of malondialdehyde (MDA) in a variety of samples including tissue, cells and plasma