Application

Ethanol Assay Kit has been used : to measure the levels of ethanol in yeast cell culture supernatant and to study the link between metabolism and chromatin structure to determine the ethanol levels in compound beverages and foods to measure ethanol content in vinegar

General description

Ethanol is a psychoactive component of many commonly consumed drinks where it acts as a central nervous system depressant. After ingestion, ethanol is absorbed into the bloodstream via the stomach and small intestine. Ethanol is largely metabolized by the liver but is also secreted in urine or through respiration. The monitoring of ethanol levels is also important in fermentation processes. The ethanol assay kit provides a simple and reliable method for the quantification of ethanol.This kit is highly sensitive to the presence of short-chain alcohols (ethanol, methanol, and propanol). Storage or use of this kit in the vicinity of alcohol vapors can result in the uptake of the alcohols by kit components, resulting in very high backgrounds.

Principle

Ethanol concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λ_ex = 535/λ_ex = 587 nm) product, proportional to the ethanol present. The Ethanol Assay Kit provides a simple and reliable method for the quantification of ethanol in serum, plasma, and other body fluids as well as in beverages and growth media.

Suitability

Suitable for determining the concentration of ethanol in serum, plasma, and other body fluids as well as in beverages and growth media.