Application

NADP/NADPH (nicotinamide adenine dinucleotide phosphate) Quantitation Kit has been used for quantification of NADP/NADPH. It has been used to quantify NAD(P)H in order to determine SHMT2 (serine hydroxymethyltransferase 2) specific enzyme activity.

General description

Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the Pentose Phosphate Pathway (PPP) is the major source of NADPH produced in animal cells.

Principle

The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NADP (nicotinamide adenine dinucleotide phosphate) and NADPH, and their ratio. There is no requirement to purify NADP/NADPH from samples. This assay is specific for NADP and NADPH and does not detect NAD or NADH. NADPt (NADP and NADPH) or NADPH are quantified in a colorimetric assay (450 nm).

Suitability

Suitable for the detection of NADP, NADPH and their ratio in a variety of biological samples including cell and tissue lysates