Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Biochem/physiol Actions

The gene PREB (prolactin regulatory element binding) encodes a type II transmembrane protein. It binds to the Pit1-binding element of the prolactin (PRL) promoter and plays a transcriptional regulatory role in the pituitary and other organs in which the protein is expressed. In response to cytokines, PREB transcriptionally regulates MCP-1 (monocyte chemoattractant protein-1) promoter. MCP-1 functions as an initiating factor in the inflammatory process of atherogenesis. PREB also functions as a guanine-nucleotide exchange factor for Sar1 guanosine triphosphatase and is essential for collagen VII secretion. It is recruited to ER (endoplasmic reticulum) exit sites by its interaction with a collagen cargo receptor component, cTAGE5. The export of large cargo at the ER exit sites involves high levels of guanosine triphosphate-bound Sar1 generated by PREB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.Every Prestige Antibody is tested in the following ways: IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. Protein array of 364 human recombinant protein fragments.

General description

The gene PREB (prolactin regulatory element binding) is also referred to as Sec12 and is mapped to human chromosome 2p23. The encoded 417 amino acids protein contains highly conserved seven WD-40 folds. It is localized to the ER (endoplasmic reticulum) exit sites in mammalian cells. The encoded protein contains two PQ-rich potential transactivation domains and lacks a DNA binding motif. This gene overlaps the αβhydrolase domain containing 1 (ABHD1) gene along a stretch of 42bp and may be associated with some of the developmental abnormalities found in patients with partial trisomy 2p.

Immunogen

Prolactin regulatory element-binding protein recombinant protein epitope signature tag (PrEST)

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

Linkage

Corresponding Antigen APREST72889.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide