AUTOSEQ G-50

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Application

illustra^™ Autoseq^™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results since residual dye-terminators can obscure data.llustra Autoseq^™ G-50 consists of MicroSpin^™ columns containing Sephadex^® G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.illustra^™ Autoseq^™ G-50 columns can be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems.

Features and Benefits

For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated sequencing. Columns are prepacked with Sephadex^® G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative. Columns that are ready to use enable sample application to collection of purified product in less than 4 min.

General description

illustra^™ Autoseq^™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results.

Legal Information

AutoSeq is a trademark of Cytiva

MicroSpin is a trademark of Cytiva

illustra is a trademark of Cytiva

Sephadex is a registered trademark of Cytiva