Application

Monoclonal Anti-Golgi 58K Protein/Formiminotransferase Cyclodeaminase (FTCD) antibody produced in mouse has been used in western blot analysis and dual immunofluorescence staining.

Monoclonal Anti-Golgi 58K Protein/Formiminotransferase Cyclodeaminase (FTCD) antibody produced in mouse is suitable for electron microscopy, indirect immunofluorescence at a working dilution of 1:50 using cultured CHO cells and indirect immunoblotting at 1:5000 working dilution using whole rat liver extract. It is also useful for the localization of Golgi 58K protein using immunoblotting, dot blot, electron microscopy, and immunocytochemistry.The antibody was used:for the analysis of distribution of the 58K9 protein exclusively localized in the Golgias a primary antibody in the immunofluorescence analysis in studies related to functioning of nuclear envelope protein TMEM209 in lung carcinoma cells, tracking TG2 (Transglutaminase Type 2) transport in renal tubular epithelial cells, binding of TRADD (TNFR-associated death domain protein) to TNF-R1 at the plasma membrane and localization of Wilson disease protein in the Golgi apparatusin immunoprecipitation studies

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunofluorescence (1 paper)Western Blotting (1 paper)

Biochem/physiol Actions

Golgi 58K protein is associated with the Golgi apparatus peripherally and has been identified as a version of FTCD (Formiminotransferase Cyclodeaminase). It is a bifunctional metabolic enzyme involved in conversion of histidine to glutamic acid. It acts as a channel for the transport of one-carbon units from formiminoglutamate to the folate pool. It also interacts with vimentin subunits and with polymerized vimentin filaments. Autosomal recessive disorder glutamate formiminotransferase deficiency is caused by defects in FTCD.

FTCD/58K protein of the Golgi apparatus used in conjunction with other antibodies to Golgi proteins (e.g., the Golgi β-COP protein) may be used for studies on the role and relationships of this protein in the cell. It is also useful for localization studies following subcellular fractionation procedures.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

FTCD is a homooctamer with its subunits arranged in a planar ring. It is predominantly expressed in the liver. Apart from being associated with the Golgi apparatus, it is detectable in the supernatant cytosolic fraction, and may also be localized to cytoplasmic vesicles.

Monoclonal Anti-Golgi 58K Protein/formiminotransferase cyclodeaminase (FTCD) (mouse IgG1 isotype) is derived from the 58K-9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with FTCD/Golgi 58K protein purified from rat liver.

Immunogen

Golgi 58K protein from rat liver.

Specificity

The antibody recognizes an epitope located on the microtubule-binding peripheral Golgi membrane 58 kDa protein. It is also useful for studies on the effect of microtubule-perturbing agents on the Golgi apparatus.