Application

Functional Genomics/Target Validation Epigenetic Modification Transcriptional Activation

Components

1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).

Features and Benefits

Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.

General description

OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.

Legal Information

CRISPR Use License AgreementLentiviral and WPRE License Agreements

Linkage

To order U6-gRNA plasmids, please visit sigma.com/crisprsClick here for more information on dcas9-p300 Gene Activator Expression Plasmid.

Other Notes

Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.