Application

Functional Genomics/Screening/Target Validation Positive control, HPRT1, CRISPR high titer lentivirus format, for creating gene knockouts/genetic modifications in cell lines.To see more application data, protocols, vector maps visit sigma.com/crispr.

Features and Benefits

Serves as a positive experimental control for the CRISPR editing workflow using lenti CRISPR DNA. The number of cells with HPRT1 knocked out increases over time with 6−TG selection.

Legal Information

CRISPR Use License AgreementLentiviral and WPRE License Agreements

Other Notes

This product is for R&D;use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL−2) organisms in laboratory handling. Follow all published RGL−2 guidelines for laboratory handling and waste decontamination.

Physical form

200 µl virus, minimum p24 titer > 5 x 10⁸ TU/mL