Application

Primary Human Renal Cortical Epithelial Cells (HRCEpC) are isolated from the cortex of the human kidney and are provided in a cryopreserved format. HRCEpC stain positive for cytokeratin. The renal cortex is the outer portion of the kidney between the renal capsule and the renal medulla. It contains the renal corpuscles, the proximal and distal convoluted tubules, and the cortical collecting ducts. Important physiological processes like osmoregulation and excretion can be studied with HRCEpC in vitro.

Cell Line Origin

Renal Cortex

Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm²Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Quality

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Subculture Routine

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Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.