Application

Human Small Airway Epithelial Cells (HSAEpC) may be used: in overexpression of α_vβ₆ integrinin cytotoxicity studies with Cannabis sativa oil extractin transfection studies with highly pathogenic avian influenza virus and influenza A virus

Cell Line Origin

Bronchiole

General description

Human Small Airway Epithelial Cells (HSAEpC) serve as model cell culture systems for in vitro immune response studies. The 3D culture of HSAEpC mimics the in vivo cells effectively. The 3D cells are more viable, functional and efficient for immune response studies compared to the 2D cells.

Primary Human Small Airway Epithelial Cells (HSAEpC) are isolated from the distal portion of the human respiratory tract in the 1 mm bronchiole area and are provided in a cryopreserved format. They stain positive for cytokeratin.The distal respiratory tract mainly consists of pulmonary alveoli, which are spherical outcroppings of the respiratory bronchioles and are the primary sites of gas exchange with the blood. HSAEpC are qualified for functional studies to investigate disorders such as asthma or pulmonary inflammation.

Other Notes

Recommended Plating Density: 10000 - 15000 cells per cm²Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: > 15

Quality

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Subculture Routine

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Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.