Application

Primary Human Coronary Artery Smooth Muscle Cells (HCASMC) are isolated from human coronary arteries and are provided in a cryopreserved format. HCASMC stain positive for smooth muscle α-actin. Coronary arteries supply the heart with blood and obstruction in atherosclerosis causes myocardial infarction.HCASMC are qualified to study the function of smooth muscle cells in the development of atherosclerotic plaques or in other diseases like hypertension. They are also useful for stent-graft compatibility testing.

Cell Line Origin

Artery

Other Notes

Recommended Plating Density: 7500 - 10000 cells per cm²Passage After Thawing: P2Tested Markers: Smooth muscle alpha-actin positiveGuaranteed population doublings: >15

Quality

Rigid quality control tests are performed for each lot of Human Smooth Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. smooth muscle a-actin, are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Subculture Routine

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Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.