Application

CelLytic^™ B Cell Lysis Reagent has been used for cell lysis for the extraction of coenzyme Q10, recombinant Src kinase associated phosphoprotein 1- glutathione S-transferases (GST) fusion protein and human α-synuclein.

A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.

Features and Benefits

Higher protein extraction efficiency than traditional methods such as sonication and lysozyme Scalable for 1 to 25 grams of bacterial cell paste No interference with downstream applications such as affinity chromatography, IP, and Western blotting Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Detergent blend formulated in 40 mM Trizma^® HCl (pH 8.0).