Application

Detect NeuN using this Anti-NeuN Antibody validated for use in Western Blotting, IHC(P), ICC.

Immunohistochemistry Analysis: A 1:3,000 dilution from a representative lot detected NeuN in normal mouse cerebellum and frontal cortex tissues.

Immunocytochemistry Analysis: A 1:8,000 dilution from a representative lot detected NeuN in Rat E18 cortex and hippocampus primary cultures.

General description

ABN90 is a Guinea Pig polyclonal version of the Anti-NeuN, clone A60 (MAB377), a highly characterized and cited mouse monoclonal antibody that specifically recognizes the DNA-binding, neuron-specific protein NeuN, which is present in most CNS and PNS neuronal cell types of all vertebrates tested. NeuN protein distributions are apparently restricted to neuronal nuclei, perikarya and some proximal neuronal processes in both fetal and adult brain although, some neurons fail to be recognized by NeuN at all ages: INL retinal cells, Cajal-Retzius cells, Purkinje cells, inferior olivary and dentate nucleus neurons, and sympathetic ganglion cells are some examples. Immunohistochemically detectable NeuN protein first appears at developmental timepoints that correspond with the withdrawal of the neuron from the cell cycle and/or with the initiation of terminal differentiation of the neuron. Immunoreactivity appears around E9.5 in the mouse neural tube and is extensive throughout the developing nervous system by E12.5. Strong nuclear staining suggests a nuclear regulatory protein function; however, no evidence currently exists as to whether the NeuN protein antigen has a function in the distal cytoplasm or whether it is merely synthesized there before being transported back into the nucleus. No difference between protein isolated from purified nuclei and whole brain extract on immunoblots has been found.

Physical form

Guinea pig polyclonal serum containing 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse brain E16 tissue lysate.

Western Blot Analysis: A 1:4,000 dilution of this antibody detected NeuN in 10 µg of mouse brain E16 tissue lysate.

Target description

~48 kDa observed