Anti-SATB1

Analysis Note

ControlA20 cell lysate

Application

Research Sub CategoryTranscription Factors

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected SATB1 in human brain tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected SATB1 in human brain cells.

Detect Special AT-rich sequence-binding protein 1 using this rabbit polyclonal antibody, Anti-SATB1 Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Special AT-rich sequence-binding protein 1 (SATB1) contains 2 CUT DNA-binding domains and one homeobox DNA binding domain belonging to the CUT homeobox family. SATB1 contributes to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma. SATB1 is organized into a cage-like network anchoring loops of heterochromatin and regulates gene expression through chromatin remodeling/HDAC (histone deacetylase complex), and transcription factors recruitment. SATB1 is predominantly expressed in thymus. Recent studies show that SATB1 is necessary for breast cancer cells to become metastatic.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human SATB1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in A20 cell lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected SATB1 in 15 µg of A20 cell lysate.

Specificity

This antibody recognizes the C-terminus of SATB1.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~90 and ~98 kDa observed. Uniprot describes two isoforms at ~86 kDa and ~89 kDa Uncharacterized bands may appear in some lysate(s).