Application

Brain PI(4,5)P₂ has been used in the preparation of liposomes for dynamin-catalyzed fission assay and as a constituent in the lipid mixture for dual polarisation interferometry(DPI)

Biochem/physiol Actions

Phosphatidyl inositol (4,5) bisphosphate (PI(4,5)P₂) plays a key role in the early stages of vesicle formation. It functions in the recruitment of endocytic coats by binding to variety of proteins involved in endocytic trafficking. PI(4,5)P₂ is hydrolysed by phospholipase C to yield the signaling molecules diacylglycerol (DAG) and phosphatidyl inositol (3,4,5) triphosphate (PI(3,4,5)P₃). PI(4,5)P₂ is required for the functioning of many ion channels.

General description

Phosphatidyl inositol (4,5) bisphosphate (PI(4,5)P₂)/PIP₂ is produced from phosphatidyl inositol monophosphates (PIP).

Although PI(4,5)P2 is a minor component of cell membranes, it plays a critical role as a substrate for a number of important signaling proteins. PI(4,5)P2 is an intermediate in the IP3/DAG pathway where it is hydrolyzed by phospholipase C to liberate the second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI(4,5)P2 is also a substrate for PI 3-kinase where it is phosphorylated to PI(3,4,5)P3, an activator of downstream signaling components such as the protein kinase AKT.

Packaging

5 mL Clear Glass Sealed Ampule (840046X-5mg)

5 mL Clear Glass Sealed Ampule (840046X-10mg)

5 mL Clear Glass Sealed Ampule (840046X-25mg)

5 mL Clear Glass Sealed Ampule (840046X-1mg)