BRAIN PI(4;5)P2

Application

Brain PI(4,5)P2 has been used:in the preparation of Folch liposomein the preparation of giant unilamellar vesicles and multilamellar lipid sheetsas a substrate for rubidium flux assayin the biochemical reaction assay mixture for various lipid kinases like Phosphatidylinositol-3-kinase gamma (PI3K_γ), Phosphatidylinositol-3-kinase beta (PI3K_β), Phosphatidylinositol-3-kinase gamma (PI3K_α)

Biochem/physiol Actions

Phosphatidylinositol 4,5-bisphosphate (PIP₂) is a signaling phospholipid, present in the plasma membrane. It acts as a precursor for inositide 3 phosphate, diacylglycerol. PIP₂ is a gating ligand for most of the ion channels. It functions as an activatory ligand for eukaryotic inward rectifier potassium (Kir) channels.

General description

Phosphatidylinositol 4,5-bisphosphate (PIP₂) is synthesized from the precursor phosphatidylinositol 4-phosphate(PI4P). PIP₂ is a phosphoinositide with three negative charges.

Although PI(4,5)P2 is a minor component of cell membranes, it plays a critical role as a substrate for a number of important signaling proteins. PI(4,5)P2 is an intermediate in the IP3/DAG pathway where it is hydrolyzed by phospholipase C to liberate the second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI(4,5)P2 is also a substrate for PI 3-kinase where it is phosphorylated to PI(3,4,5)P3, an activator of downstream signaling components such as the protein kinase AKT.

Packaging

5 mL Clear Glass Sealed Ampule (840046P-25mg)

5 mL Clear Glass Sealed Ampule (840046P-10mg)

5 mL Clear Glass Sealed Ampule (840046P-5mg)

5 mL Clear Glass Sealed Ampule (840046P-1mg)