General description

A Wnt-5a-derived, N-terminally butyloxycarbonyl- (Boc) protected, hexa-peptide that effectively antagonizes Wnt-5a-mediated cellular activities in A2058 and HTB63 melanoma cultures, including migration, invasion, calcium response, and MARCKS phosphorylation. Replacing the N-terminal Boc with a formyl modification results in a peptide termed Foxy5 that mimics, instead of antagonizes, Wnt-5a biological activity in a Frizzled-5- (Fzd-5/Frz-5) dependent manner. The peptide sequence is conserved in multiple species, including human, mouse, and rat, and Box5 is expected to exert its effect via direct FZD5 binding as is the case with Foxy5.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Jenei, V., et al. 2009. Proc. Natl. Acad. Sci. USA106, 19473.Safholm, A., et al. 2006. J. Biol. Chem.281, 2740.

Packaging

Packaged under inert gas

10 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Sequence

t-Boc-NH-Met-Asp-Gly-Cys-Glu-Leu-CO₂H

Warning

Toxicity: Standard Handling (A)