Cell Line Description

DerivationPrimary cell type: Fibroblast of dermisPassage number reprogrammed: 25Reprogramming methodVector type: IntegratingVector: VirusVirus type: LentivirusGene list: KLF4MYCPOU5F1SOX2Have the reprogramming vectors been silenced: YesMerthods used: rtpcrCharacterizationAnalysis of Undifferentiated CellsMarker expression: SSEA-1 (-)TRA 1-60 (+)SSEA-4 (+)Differentiation potencyEctoderm: BTUBULIN (-)FOXG1(-)HES5(+)NEUROD1(+)PAX6(-)Sox1(+)Endoderm: CXCR4 (+)FOXA2(-)GATA6(+)GSC (-)SOX17(+)Mesoderm: BMP4(+)DECORIN (+)GATA4(-)HAND1(+)PDGFRA(+)PECAM1(+)PITX1(-)VIMENTIN(+)Microbiology / Virus ScreeningHIV 1: NegativeHIV 2: NegativeHepatitis B: NegativeHepatitis C: NegativeMycoplasma: NegativeSterilityInoculation for microbiological growth: No Contaminants DetectedMycoplasma: Not DetectedViability: Viable post-cryopreservationGenotypingKaryotypingPassage number: 37Cell line karyotype: 46,XYKaryotyping method: G-BandingGenotypingSTR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Cell Line Origin

DepositorThe Hubrecht Institute

General description

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer’;s Disease, Parkinson’;s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington’;s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

Legal Information

mTeSR is a registered trademark of WiCell Research Institute, Inc.

EBiSC is a trademark of Fraunhofer-Gesellschaft

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

Linkage

Additional Cell Line Info EBiSC Access Use Agreement (EAUA) Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

Subculture Routine

Medium: mTeSR^®Passage method: EDTAMatrix: Matrigel^® / Geltrex^®CO₂ concentration: 5%O₂ concentration: 20%Temperature: 37°C